The ability to synthesize oligonucleotides with specific sequences using phosphoramidite chemistry has revolutionized molecular diagnostics. Here are some examples of how oligonucleotides are used in diagnostics:

 

Polymerase Chain Reaction (PCR): PCR is a technique used to amplify a specific DNA sequence. Oligonucleotides called primers are used to initiate DNA synthesis at the flanking regions of the target sequence.

 

Southern Blotting: Southern blotting is a technique used to detect specific DNA sequences in a sample. Oligonucleotides called probes are used to bind to the target DNA sequence by complementary base pairing.

 

Northern Blotting: Northern blotting is similar to Southern blotting, but it is used to detect specific RNA sequences.

 

Western Blotting: Western blotting is used to detect specific proteins in a sample. While not directly involving oligonucleotides, some western blot techniques utilize oligonucleotide probes labelled with antibodies for signal amplification.

 

In Situ Hybridization (ISH): ISH is a technique used to visualize the location of specific DNA or RNA sequences within cells or tissues. Oligonucleotide probes are used to bind to the target sequence and are then visualized using a fluorescent label.

 

DNA Microarrays: DNA microarrays are used to analyze the expression of thousands of genes simultaneously. Oligonucleotides are spotted onto a solid surface in a high-density array, and then the sample being analyzed is hybridized to the array. The pattern of hybridization can be used to identify genes that are differentially expressed.

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PD1-004.svg

DMT-dA(Bz)-CE-Phosphoramidite

目录号: PD1-004
分子式: C47H52N7O7P
CAS 号: 98796-53-3
PD2-004.svg

DMT-dG(iBu)-CE-Phosphoramidite

目录号: PD2-004
分子式: C44H54N7O8P
CAS 号: 93183-15-4
PD3-007.svg

DMT-dC(Ac)-CE-Phosphoramidite

目录号: PD3-007
分子式: C41H50N5O8P
CAS 号: 154110-40-4
PD4-002.svg

DMT-dT-CE-Phosphoramidite

目录号: PD4-002
分子式: C40H49N4O8P
CAS 号: 98796-51-1
PD5-002.svg

DMT-dU-CE-Phosphoramidite

目录号: PD5-002
分子式: C39H47N4O8P
CAS 号: 109389-30-2
PD6-002.svg

DMT-dI-CE-Phosphoramidite

目录号: PD6-002
分子式: C40H47N6O7P
CAS 号: 141684-35-7
PR3-008.svg

DMT-2'-O-TBDMS-C(Ac)-CE-Phosphoramidite

目录号: PR3-008
分子式: C47H64N5O9PSi
CAS 号: 121058-88-6
PR2-007.svg

DMT-2'-O-TBDMS-G(iBu)-CE-Phosphoramidite

目录号: PR2-007
分子式: C50H68N7O9PSi
CAS 号: 147201-04-5
PR5-003.svg

DMT-2'-O-TBDMS-U-CE-Phosphoramidite

目录号: PR5-003
分子式: C45H61N4O9PSi
CAS 号: 118362-03-1
PR6-003.svg

DMT-2'-O-TBDMS-I-CE-Phosphoramidite

目录号: PR6-003
分子式: C46H61N6O8PSi
PR1-001.svg

2'-OMe-Bz-A-CE-Phosphoramidite

目录号: PR1-001
分子式: C48H54N7O8P
CAS 号: 110782-31-5
PR2-002.svg

2'-OMe-iBu-G-CE-Phosphoramidite

目录号: PR2-002
分子式: C45H56N7O9P
CAS 号: 150780-67-9
PR3-001.svg

DMT-2'-OMe-C(Ac)-CE-Phosphoramidite

目录号: PR3-001
分子式: C42H52N5O9P
CAS 号: 199593-09-4
PR5-001.svg

2'-OMe-U-CE-Phosphoramidite

目录号: PR5-001
分子式: C40H49N4O9P
CAS 号: 110764-79-9
PD1-001.svg

DMT-2'-F-dA(Bz)-CE-Phosphoramidite

目录号: PD1-001
分子式: C47H51FN7O7P
CAS 号: 136834-22-5
PD3-001.svg

2'-F-Ac-dC-CE-Phosphoramidite

目录号: PD3-001
分子式: C41H49FN5O8P
CAS 号: 159414-99-0
PD2-002.svg

DMT-2'-F-dG(iBu)-CE-Phosphoramidite

目录号: PD2-002
分子式: C44H53FN7O8P
CAS 号: 144089-97-4
PD6-001.svg

2'-F-dI-CE-Phosphoramidite

目录号: PD6-001
分子式: C40H46FN6O7P
PD5-001.svg

2'-F-dU-CE-Phosphoramidite

目录号: PD5-001
分子式: C39H46FN4O8P