Linkers play a vital role in oligo synthesis, specifically in two main applications:

 

Solid Phase Attachment: During oligonucleotide synthesis, a linker molecule covalently attaches the growing oligonucleotide chain to a solid support. This solid support is typically a controlled-pore glass (CPG) bead. The linker offers a stable bond to the solid phase, allowing for the stepwise addition of nucleotides to create the desired oligonucleotide sequence. After synthesis is complete, the linker is cleaved under specific conditions, releasing the purified oligonucleotide from the solid support.

 

Bioconjugation: Linkers can also be incorporated at either the 5' or 3' terminus of the oligonucleotide to facilitate conjugation with various biomolecules. These biomolecules can include fluorophores, quenchers, antibodies, nanoparticles, or other functional groups. Bioconjugation allows researchers to attach functionalities to the oligonucleotide, enabling diverse applications such as:
• Fluorescence in situ hybridization (FISH) for cellular imaging
• Detection of specific nucleic acid sequences
• Immunoassays
• Drug delivery

 

There are various types of linkers available for oligo synthesis, each with unique properties and functionalities. Some common linker types include:


• Cleavable Linkers: These linkers are designed to be cleaved from the solid support under specific conditions, such as exposure to acid or base. This allows for the release of the purified oligonucleotide.
• Non-Cleavable Linkers: These linkers form a permanent bond with the solid support. While the oligonucleotide remains tethered to the solid phase, non-cleavable linkers are useful for applications like microarrays where the oligos stay attached to the surface.
• Biotinylated Linkers: These linkers contain biotin, a small molecule that can bind to streptavidin with high affinity. This property allows for easy purification of the oligonucleotide using streptavidin-coated magnetic beads.
• Fluorescent Linkers: These linkers incorporate a fluorescent moiety, enabling the direct detection of the labeled oligonucleotide.

 


The choice of linker in oligo synthesis depends on the desired application and the downstream processes involved.

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ON-093.svg

6-MMT-NH-Hexanol

目录号: ON-093
分子式: C26H31NO2
ON-152.svg

N6-Tr-6-amino-hexanol

目录号: ON-152
分子式: C25H29NO
ON-130.svg

DMT-hydroxymethyl-hexyl-phthalimide

目录号: ON-130
分子式: C36H37NO6
ON-153.svg

TEG linker

目录号: ON-153
分子式: C36H37NO6
ON-154.svg

TEG Linker2

目录号: ON-154
分子式: C33H45NO8
ON-155.svg

6-N3-1-hexanol

目录号: ON-155
分子式: C6H13N3O
CAS 号: 146292-90-2
ON-156.svg

6-amino(Fmoc)-2-hydroxymethylhexan-1-ol

目录号: ON-156
分子式: C22H27NO4
ON-214.svg

DMT-Hexylamine-linker Phosphoramidite

目录号: ON-214
分子式: C36H50N3O4P
OP-065.svg

1-O-DMTr-Glycerol-TEG-Cholesterol-2-CE-Phosphoramidite

目录号: OP-065
分子式: C68H103N2O10P
ON-224.svg

Fmoc-aminopropyloxy-3-DMTr-glycerol

目录号: ON-224
分子式: C42H43NO7
ON-283.svg

TFA-EO Linker

目录号: ON-283
分子式: C7H8F3NO2
ON-267.svg

1, 3-Di-DMT Glycerol succinate, TEA salt

目录号: ON-267
分子式: C49H48O10
OP-020.svg

DMTr-Cholesteryl-TEG Phosphoramidite (plant source)

备注: Plant Source
目录号: OP-020
分子式: C70H106N3O11P
ON-371.svg

N-(Boc-aminoethyl)-Gly-oEt

目录号: ON-371
分子式: C11H22N2O4
ON-373.svg

Diethyl 2-(4-chlorobutyl)malonate

目录号: ON-373
分子式: C11H19ClO4
OP-064.svg

DFA-AH Linker Amidite

目录号: OP-064
分子式: C17H32F2N3O3P
OP-055.svg

Phenylacetyl HA amidite linker

目录号: OP-055
分子式: C23H38N3O3P
ON-173.svg

Fmoc-AEG-OMe•HCl

目录号: ON-173
分子式: C20H23ClN2O4
ON-172.svg

Fmoc-AEG-OH•HCl

目录号: ON-172
分子式: C19H21ClN2O4
CAS 号: 172405-44-6
ON-379.svg

Ethyl-γ-(N-(3-hydroxypropyl)anilino)-butyrate

目录号: ON-379
分子式: C15H23NO3
ON-380.svg

Propargyl-PEG2-NH2

目录号: ON-380
分子式: C7H13NO2
ON-381.svg

6-amino(Fmoc)-1-O-DMT-2-hydroxymethylhexan-1-ol

目录号: ON-381
分子式: C43H45NO6
ON-382.svg

N-Fmoc-2-(2-aminoethoxy)ethanol

目录号: ON-382
分子式: C19H21NO4
D4-034.svg

5'-DMT-dT-linker-NH2

目录号: D4-034
分子式: C39H46N4O8
ON-417.svg

Cholesteryl succinate, TEA salt (plant source)

备注: Plant Source
目录号: ON-417
分子式: C61H86O9 (free acid)
ON-419.svg

Cholesterol tetraethylene glycol (TetraEG) amidite (plant source)

备注: Plant Source
目录号: ON-419
分子式: C48H86N3O8P
ON-420.svg

Cholesteryl-TEG-succinate (plant source)

备注: Plant Source
目录号: ON-420
分子式: C65H93NO13 (free acid)
CAS 号: 873435-28-0
ON-422.svg

Cholesteryl amidite (plant source)

备注: Plant Source
目录号: ON-422
分子式: C68H103N2O10P
ON-423.svg

Cholesterol-TEG CPG 500Å (plant source)

备注: Plant Source
目录号: ON-423
ON-424.svg

Cholesterol-TEG CPG 1000Å (plant source)

备注: Plant Source
目录号: ON-424
ON-421.svg

Cholesterol-TEG

目录号: ON-421
分子式: C61H89NO10
CAS 号: 869354-69-8
OP-168.svg

n-Butyl-2-cyanoethyl-N, N-diisopropylphosphoramidite

目录号: OP-168
分子式: C13H27N2O2P
OP-171.svg

MMTr C3 linker Phosphoramidite

目录号: OP-171
分子式: C32H42N3O3P
OP-172.svg

NHMMTr-C4 Phosphoramidite

目录号: OP-172
分子式: C33H44N3O3P
OP-173.svg

NHMMTr-C5 Phosphoramidite

目录号: OP-173
分子式: C34H46N3O3P
OP-174.svg

NHMMTr-C7 Phosphoramidite

目录号: OP-174
分子式: C36H50N3O3P
OP-182.svg

Spacer C12 Phosphoramidite

目录号: OP-182
分子式: C42H61N2O5P
CAS 号: 158665-27-1
OP-189.svg

1, 3-di-O-DMTr-glycerol-bis-(diisopropylamino)-Phosphane

目录号: OP-189
分子式: C57H71N2O7P
OP-190.svg

6-O-DMTr-hexane-bis-(diisopropylamino)-Phosphane

目录号: OP-190
分子式: C39H59N2O4P
OP-191.svg

12-O-DMTr-dodecane-bis-(diisopropylamino)-Phosphane

目录号: OP-191
分子式: C45H71N2O4P
OP-192.svg

1, 3-di-O-DMTr-glycerol-CE-Phosphoramidite

目录号: OP-192
分子式: C54H61N2O8P
ON-407.svg

β-Mannose C4 alcohol

目录号: ON-407
分子式: C18H28O11
ON-408.svg

α-Mannose C4 alcohol

目录号: ON-408
分子式: C18H28O11
ON-402.svg

Galactose C4 alcohol

目录号: ON-402
分子式: C18H28O11
ON-400.svg

Glucose C4 alcohol

目录号: ON-400
分子式: C18H28O11
ON-439.svg

benzyl 6-aminohexanoate

目录号: ON-439
分子式: C20H27NO5S
ON-440.svg

Methacrylate C6

目录号: ON-440
分子式: C10H19NO2
ON-442.svg

2-(2-nitrophenyl)propyl imidazole-1-carboxylate

目录号: ON-442
分子式: C13H13N3O4
ON-449.svg

3'-thio modifier 6 S-S-DMTr-linker-succinate, TEA salt

目录号: ON-449
分子式: C35H44O9S2(free acid)
OP-175.svg

NHMMTr-C8 Phosphoramidite

目录号: OP-175
分子式: C37H52N3O3P
OP-183.svg

DMTr-hydroxymethyl hexyl-Biotin Phosphoramidite

目录号: OP-183
分子式: C47H66N5O7PS
ON-503.svg

N-[2-[(2-Aminoethyl)amino]ethyl]-1,2-dithiolane-3-pentanamide

目录号: ON-503
分子式: C12H25N3OS2
CAS 号: 2855082-29-8
ON-504.svg

N2-[5-(1,2-Dithiolan-3-yl)-1-oxopentyl]-L-arginine methyl ester

目录号: ON-504
分子式: C15H29N4O3S2+
CAS 号: 1422468-18-5
ON-529.svg

Biotin-LC-NHS Ester

目录号: ON-529
分子式: C20H30N4O6S
ON-453.svg

2, 5-dioxopyrrolidin-1-yl-3-(dodecyldisulfaneyl)propanoate

目录号: ON-453
分子式: C19H33NO4S2
ON-491.svg

Peracetylated GalNAc PEG linker-Azide

目录号: ON-491
分子式: C22H36N4O12
ON-530.svg

Peracetylated GalNAc PEG linker-Amino

目录号: ON-530
分子式: C22H38N2O12 (free acid)
ON-781

Benzyl N-[2-(Fmoc)aminoethyl]glycinate

目录号: ON-781
分子式: C26H26N2O4(free base)
CAS 号: 511534-93-3
ON-783

t-butylbenzoyl biotin-NHS ester

目录号: ON-783
分子式: C25H31N3O6S