Enzymes

 

Enzymes are biological catalysts that accelerate specific chemical reactions in a cell. In molecular diagnostics, they are used in various applications such as:

 

Nucleic Acid Amplification: Enzymes like DNA polymerases are crucial for techniques like PCR (Polymerase Chain Reaction), which amplifies specific DNA sequences for detection.

 

Restriction Digestion: Restriction enzymes cleave DNA at specific recognition sequences, creating fragments used for analysis in techniques like gel electrophoresis and Southern blotting.

 

Ligation: Ligases join DNA fragments, used in cloning and DNA modification techniques.

 

Probe Digestion: Enzymes can digest probes used in diagnostic assays after they hybridize to their target molecules.

 

Buffers

 

Buffers are solutions that maintain a constant pH in a reaction. They are essential in molecular diagnostics because many enzymes have specific pH ranges for optimal activity. Buffers also help to maintain ionic strength and prevent unwanted interactions between biomolecules. Here's how buffers are important in diagnostics:

 

Maintaining Enzyme Activity: Enzymes function best within a specific pH range. Buffers ensure this optimal pH is maintained throughout the diagnostic assay.

 

Optimizing Reaction Conditions: Buffers can influence factors like ionic strength, which can affect how biomolecules interact and how efficiently reactions proceed.

 

Standardization: Buffers with consistent compositions allow for standardized diagnostic protocols, ensuring reliable and reproducible results.

 


In conclusion, enzymes and buffers are essential tools in molecular biological diagnostics. Enzymes act as biological catalysts to drive specific reactions, while buffers provide optimal reaction conditions by maintaining pH and ionic strength. Their combined application allows for the sensitive and specific detection of diseases, genetic variations, and other biological targets.

筛选

目录下载

产品共计 0

enzymes_img1.svg

pfu DNA polymerase

目录号: ON-051
enzymes_img2.svg

100mM MgCl2

目录号: ON-013
enzymes_img2.svg

10X MnCl2

目录号: ON-079
enzymes_img2.svg

10mM GTP

目录号: ON-075
enzymes_img2.svg

50% PEG 8000

目录号: ON-112
enzymes_img2.svg

25mM MgCl2

目录号: ON-060
enzymes_img2.svg

10X EDTA

目录号: ON-080
enzymes_img2.svg

5×RT Reaction Buffer

目录号: ON-067
enzymes_img2.svg

10X pfu Reaction Buffer

目录号: ON-068
enzymes_img2.svg

10X IVT Reaction Buffer

目录号: ON-062
enzymes_img2.svg

10X Capping Buffer

目录号: ON-073
enzymes_img1.svg

RNase III, E.coli

目录号: ON-024
enzymes_img1.svg

Capped RNA Synthesis Kit

目录号: ON-257
enzymes_img1.svg

T4 RNA Ligase 1

备注: T4 Rnl 1
目录号: ON-081
enzymes_img1.svg

DNase I (RNase-free)

目录号: ON-109
enzymes_img1.svg

T4 RNA Ligase 2

备注: T4 Rnl 2
目录号: ON-544-C010
enzymes_img1.svg

RNase R

目录号: ON-509-C020
enzymes_img1.svg

Vaccinia Capping Enzyme

目录号: ON-028
enzymes_img1.svg

Taq DNA Ligase

目录号: ON-308
enzymes_img1.svg

BspQI

目录号: ON-124
enzymes_img1.svg

T4 DNA Ligase

目录号: ON-157
enzymes_img1.svg

Taq DNA polymerase

目录号: ON-007
enzymes_img1.svg

T7 RNA polymerase

目录号: ON-004
enzymes_img1.svg

Poly(A) Polymerase

目录号: ON-126
enzymes_img1.svg

BspQI (10U/ul)

规格: GMP
目录号: ON-124-G
enzymes_img1.svg

T7 RNA polymerase (50U/ul)

规格: GMP
目录号: ON-004-G
enzymes_img1.svg

T7 RNA polymerase (200U/ul)

规格: GMP
目录号: ON-004-C200-G
enzymes_img1.svg

DNase I (RNase-free) (1U/ul)

规格: GMP
目录号: ON-109-G
enzymes_img1.svg

Pyrophosphatase Inorganic (yeast) (0.1U/ul)

规格: GMP
目录号: ON-025-G
enzymes_img1.svg

Vaccinia Capping Enzyme

规格: GMP
目录号: ON-028-G
enzymes_img1.svg

2'-O-Methyltransferase (50U/ul)

规格: GMP
目录号: ON-014-G
enzymes_img1.svg

RNase Inhibitor (40U/ul)

规格: GMP
目录号: ON-039-G
enzymes_img2.svg

10X IVT Reaction Buffer

规格: GMP
目录号: ON-062-G
enzymes_img2.svg

10X Capping Buffer

规格: GMP
目录号: ON-073-G
enzymes_img2.svg

10X Cut Buffer

规格: GMP
目录号: ON-268-G
enzymes_img2.svg

S-adenosylmethionine (SAM)

规格: GMP
目录号: ON-074-G