PMO

 

 

PMO monomers, specifically phosphorodiamidate morpholino (PDM) monomers, play a vital role in the synthesis of oligonucleotides called phosphorodiamidate morpholino oligonucleotides (PMOs). PMOs are a class of antisense therapeutic agents that bind to target RNA molecules and modulate gene expression. Here's a breakdown of how PMO monomers are applied in oligo synthesis:

 

Function:

• PMO monomers act as the building blocks for PMO oligonucleotides.

• Each PMO monomer contains a morpholino ring, a linker group, and a phosphoramidate moiety.

• The morpholino ring specifically recognizes and binds to complementary RNA sequences.

• The linker group connects the morpholino ring to the phosphoramidate moiety.

• The phosphoramidate moiety plays a crucial role in linking individual PMO monomers during oligo synthesis.

 

Process:

• PMO synthesis typically employs a solid-phase approach similar to DNA synthesis.

• A controlled sequence of PMO monomers is attached to a solid support one by one.

• Techniques like Fmoc (Fluorenylmethoxycarbonyl) or trityl chemistry are used to protect and deprotect functional groups on the monomers during the coupling process.

• After chain assembly, the PMO oligonucleotide is cleaved from the solid support and deprotected to obtain the final product.

 

Advantages of PMO Monomers:

Compared to traditional DNA oligonucleotides, PMO oligonucleotides made with PMO monomers offer several advantages:

• Increased stability: PMO backbone is more resistant to enzymatic degradation by nucleases.

• Improved binding affinity: PMO oligonucleotides can exhibit higher binding affinity towards target RNA.

• Reduced toxicity: PMO oligonucleotides generally demonstrate lower off-target effects and toxicity compared to DNA counterparts.

 

Challenges and Future Directions:

Although PMO monomers offer numerous benefits, there are ongoing efforts to improve PMO synthesis:

• Optimizing deprotection and coupling methods for higher efficiency.

• Developing new PMO chemistries compatible with automated synthesizers for faster and more scalable production.

• Creating new PMO conjugates with improved delivery properties.

 

Overall, PMO monomers are a critical component in the synthesis of PMO oligonucleotides, a promising class of therapeutic agents with the potential to treat various diseases.

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PR1-007.svg

Morpholino A subunit

目录号: PR1-007
分子式: C38H37ClN7O4P
CAS 号: 956139-18-7
PR3-014.svg

Morpholino C subunit

目录号: PR3-014
分子式: C37H37ClN5O5P
CAS 号: 956139-21-2
PR4-003.svg

Morpholino T subunit

目录号: PR4-003
分子式: C31H34ClN4O5P
CAS 号: 956139-30-3
PR2-012

O(6)-NPE-N(2)-iBu protected G PMO Monomer

目录号: PR2-012
分子式: C43H46ClN8O7P
PR5-074.svg

Morpholino U

目录号: PR5-074
分子式: C30H32ClN4O5P
PR2-051.svg

Morpholino G(PhAc) subunit

目录号: PR2-051
分子式: C39H39ClN7O5P
PR1-113.svg

Morpholino A(Ceoc) subunit

目录号: PR1-113
分子式: C35H36ClN8O5P
PR3-128.svg

Morpholino C(Ceoc) subunit

目录号: PR3-128
分子式: C34H36ClN6O6P
OP-260

O(6)-NPE I PMO Monomer

目录号: OP-260
分子式: C39H39ClN7O6P